Electroporation-Based Technologies and Treatments — International Scientific Workshop & Postgraduate Course
The meeting · 10th edition
Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting — the 10th edition of EBTT.
The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought — each participant submitting an abstract was also required to give a short oral presentation of the work. This course was accredited also by the Medical Chamber of Slovenia, so participants received 14 CME credits.
Read all about EBTT in an article published by MediaResearch Ltd (2012). International Innovation is the leading global dissemination resource for the wider scientific, technology and research communities — more at www.researchmedia.eu.
University of Ljubljana · Institute of Oncology, Ljubljana
Chair: Peter Kramar
Members: Matej Kranjc, Lea Vukanović, Tadeja Forjanič, Duša Hodžić
Alumni
Caterina Merla, Ph.D.
CNRS UMR 8203, Institut Gustave Roussy, 114 Rue E. Vaillant, 94805 Villejuif, France
Dr. Luca Campana
Centro Melanoma, Istituto Oncologico Veneto (IOV), Via Gattamelata 64, 35128 Padova, Italy
Dr. Zachary Levine
Department of Chemistry and Biochemistry, University of California Santa Barbara, 552 University Rd., Santa Barbara, CA 93106, USA
www.zachlevine.com
Course content
Resting & induced transmembrane voltage; dielectric properties of tissues; temperature & conductivity changes.
Electrochemical membrane thermodynamics; molecular dynamics simulations.
Protocols for electroporation of cells in vitro and in vivo.
Devices & electrodes for in vitro / in vivo work; tissue models & their experimental validation.
Basic mechanisms, preclinical studies, clinical use, current developments & future perspectives.
Gene transfection in vitro / in vivo; clinical use in human medicine.
Electroporation for gene delivery in the skin; DNA vaccines.
Giant vesicles in fields; PEF microalgae valorization; conductivity tensor imaging; membrane diagnostics.
Faculty
Damijan Miklavčič (University of Ljubljana) · Lluis M. Mir (CNRS & Institut Gustave Roussy) · Eberhard Neumann (University of Bielefeld) · Véronique Préat (Catholic University of Louvain) · Gregor Serša (Institute of Oncology, Ljubljana) · Justin Teissié (CNRS & IPBS, Toulouse)
Hands-on
Wet-lab, computer-modelling, hardware and e-learning sessions. Participants selected up to three sessions from wet lab and modelling, plus computer simulation and e-learning. Exercise sheets (PDF) as distributed to participants.
| S1 | Laboratory safety | PDF ↓ |
| S2 | Electroporation hardware safety | PDF ↓ |
| L1 | The influence of Mg2+ ions on gene electrotransfer efficiency | PDF ↓ |
| L2 | Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM | PDF ↓ |
| L3 | Electropermeabilization detection with propidium iodide in plated cells | PDF ↓ |
| L4 | Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS | PDF ↓ |
| L5 | Electrotransformation of Escherichia coli with plasmid DNA | PDF ↓ |
| L6 | Electroporation of planar lipid bilayers | PDF ↓ |
| L7 | E. coli inactivation by pulsed electric fields in a continuous flow system | PDF ↓ |
| L8 | Analysis of electric field orientations on gene electrotransfer visualization at the membrane level | PDF ↓ |
| L9 | Skin electroporation | PDF ↓ |
| L10 | Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments | PDF ↓ |
| L11 | Monitoring of electric field distribution in potato tuber tissue by means of magnetic resonance electrical impedance tomography | PDF ↓ |
| C1 | Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage & electrode position | PDF ↓ |
| C2 | Numerical modelling of thermal effects during irreversible electroporation treatments | PDF ↓ |
| C3 | Molecular dynamics simulations of membrane electroporation | PDF ↓ |
| E1 | Electroporation of cells and tissues — interactive e-learning | PDF ↓ |
| H1 | Measurement of electroporation pulses with oscilloscope, and voltage and current probes | PDF ↓ |
| H2 | Development of pulsed power generators for electroporation | PDF ↓ |
Abstracts & publication
After presenting their work, participants were encouraged to submit a manuscript for consideration for publication in a special issue of the Journal of Membrane Biology, devoted to electroporation-based technologies and treatments, guest-edited by P. T. Vernier, L. M. Mir and D. Miklavčič. Participation to the workshop included attendance to lectures, book of abstracts and course material, lunches and social events, and certificate of attendance and examination (5 ECTS and 14 CME if required).
Electronic proceedings — all abstracts and course material.
Download proceedings (PDF)Photo gallery
Getting here
Held at the Faculty of Electrical Engineering, University of Ljubljana. Faculty floorplan (PDF)
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